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KMID : 0385020020180020068
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Korean Journal of Laboratory Animal Science
2002 Volume.18 No. 2 p.68 ~ p.72
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Selenium Localization Pattern in The Mouse Testes Treated with Sodium Selenite
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Jang Mi-Youn
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Abstract
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Selenium localization was demonstrated in the mice testes by a histochemical technique. Sodium selenite (20 ㎎/㎏ body weight) was treated to 8-week-old ICR mice by a single intraperitoneal injection and then its deposits were observed in the seminiferous tubules using a physical developer after 1 hour of injection. In testes, selenium particles were mainly observed near to the cell membrane of spermatogenic cells and in the cytoplasm of Leydig cells. During spermatogenesis, selenium deposits first appeared in the type A spermatogonia of stage Ⅰ and greatly increased in type B spermatogonia of stage Ⅵ and preleptotene spermatocytes of stages Ⅶ and Ⅷ, but only a small number of the deposits were observed in leptotene and zygotene spermatocytes of stages Ⅸ-XII. The selenium particles were greatly increased in the pachytene spermatocytes of stages Ⅰ-Ⅴ, but they gradually began to decrease in the spermatocytes after stage Ⅵ and were not observed in spermatids after the secondary meiosis. On the other hand, a lot of selenium particles were observed in Leydig cells, while they were not detected in Sertoli cells and peritubular myoid cells. These findings suggest that selenium may be mainly related to the early phase of spermatogenesis such as the differentiation phase of spermatogonia and meiotic phase of spermatocytes.
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